A sensitive radiometric assay for cysteic acid decarboxylase activity in crude enzyme preparations of rat liver and brain. |
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| Authors: | C F Yam G Tunnicliff T T Ngo A Barbeau |
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| Affiliation: | Clinical Research Institute of Montreal, Quebec, Canada. |
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| Abstract: | 1. A method is described for the synthesis of L-[U-14C]cysteic acid from L-[U-14C] cysteine hydrochloride and for its subsequent utilisation as a substrate for cysteic acid decarboxylase activity in liver and brain. 2. The enzyme determination relies on the entrapment of radio-labelled carbon dioxide in Hyamine hydroxide. 3. The assay is sensitive, reliable and convenient and is particularly suitable for measuring the activity of the decarboxylase in crude enzyme preparations. |
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