Multiple activities of insect repellents on odorant receptors in mosquitoes |
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Authors: | Bohbot J D Fu L LE T C Chauhan K R Cantrell C L Dickens J C |
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Institution: | Invasive Insect Biocontrol and Behavior Laboratory, Plant Sciences Institute, U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Henry A. Wallace Beltsville Agricultural Research Center, Beltsville, MD, U.S.A. joseph.dickens@ars.usda.gov |
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Abstract: | Several lines of evidence suggest that insect repellent molecules reduce mosquito-host contacts by interacting with odorants and odorant receptors (ORs), thereby ultimately affecting olfactory-driven behaviours. We describe the molecular effects of 10 insect repellents and a pyrethroid insecticide with known repellent activity on two highly specific Aedes aegypti (Diptera: Culicidae) ORs, AaOR2 + AaOR7 and AaOR8 + AaOR7, exquisitely sensitive to key mosquito attractants indole and (R)-(-)-1-octen-3-ol, expressed in oocytes of Xenopus (Anura: Pipidae). Our study demonstrates that insect repellents can both inhibit odorant-evoked currents mediated by ORs and independently elicit currents in the absence of odorants. All of the repellents had effects on one or both ORs; most of these compounds were selective inhibitors and showed a high degree of specificity in their capacity to activate the two ORs. These results show that a range of insect repellents belonging to structurally diverse chemical classes modulate the function of mosquito ORs through multiple molecular mechanisms. |
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Keywords: | Aedes aegypti agonist antagonist indole modulator mosquito octenol odorant receptor pyrethroid repellent |
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