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Chromobodies to Quantify Changes of Endogenous Protein Concentration in Living Cells

Affiliation:	3. Pharmaceutical Biotechnology, Eberhard Karls University Tuebingen, Germany;4. Natural and Medical Sciences Institute at the University of Tuebingen, Reutlingen, Germany

Abstract:	Download : Download high-res image (90KB) Download : Download full-size image Highlights •Chromobodies are stabilized by antigen binding in live cells. •Monitoring changes of endogenous protein levels in living cells with chromobodies. •Broadly applicable system to generate turnover-accelerated chromobodies. •Quantification of time- and dose-dependent compound effects.

Keywords:	Antibodies Cell biology Fluorescence Imaging Imaging Visualization Tools Molecular Probes N-terminal modifications Protein Degradation Protein Turnover Ubiquitin chromobody nanobody
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