Autocrine stimulation of IL-10 is critical to the enrichment of IL-10-producing CD40hiCD5+ regulatory B cells in vitro and in vivo |
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| Authors: | Hyuk Soon Kim Jun Ho Lee Hee Dong Han A-Ram Kim Seung Taek Nam Hyun Woo Kim Young Hwan Park Dajeong Lee Min Bum Lee Yeong Min Park Hyung Sik Kim Young Mi Kim Ji Chang You Wahn Soo Choi |
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| Institution: | 1.School of Medicine, Konkuk University, Chungju 380-701;2.College of Medicine, Korea University, Seoul 136-701;3.College of Pharmacy, Sungkyunkwan University, Suwon 440-746;4.College of Pharmacy, Duksung Women’s University, Seoul 132-714;5.National Research Laboratory of Molecular Virology, Department of Pathology, School of Medicine, The Catholic University of Korea, Seoul 137-701, Korea |
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| Abstract: | IL-10-producing B (Breg) cells regulate various immune responses. However, their phenotype remains unclear. CD40 expression was significantly increased in B cells by LPS, and the Breg cells were also enriched in CD40hiCD5+ B cells. Furthermore, CD40 expression on Breg cells was increased by IL-10, CD40 ligand, and B cell-activating factor, suggesting that CD40hi is a common phenotype of Breg cells. LPS-induced CD40 expression was largely suppressed by an anti-IL-10 receptor antibody and in IL-10−/−CD5+CD19+ B cells. The autocrine effect of IL-10 on the CD40 expression was largely suppressed by an inhibitor of JAK/STAT3. In vivo, the LPS treatment increased the population of CD40hiCD5+ Breg cells in mice. However, the population of CD40hiCD5+ B cells was minimal in IL-10−/− mice by LPS. Altogether, our findings show that Breg cells are largely enriched in CD40hiCD5+ B cells and the autocrine effect of IL-10 is critical to the formation of CD40hiCD5+ Breg cells. BMB Reports 2015; 48(1): 54-59] |
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| Keywords: | Autocrine CD5 CD40 Interleukin-10 Regulatory B cells |
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