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A Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid,Ultra-robust Proteomics
Institution:3. Evosep Biosystems, Odense, Denmark;4. Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany;5. Novo Nordisk Foundation Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
Abstract:
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  • Highlights
    • •Pre-formed and offset gradients for high throughput, robustness and peptide re-focusing.
    • •Minimal cross-contamination by disposable trap columns and partial elution.
    • •Single shot DIA measurements achieve >5000 proteins in 21 min.
    Keywords:Mass Spectrometry  HPLC  Automation  Clinical proteomics  High Throughput Screening  Pre-formed gradient  Robustness  StageTip
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