Concentration and purification of two small RNA viruses: mycophage PS-1 and bacteriophage phi6 |
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Authors: | R M Van Frank W J Kleinschmidt |
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Institution: | Eli Lilly and Company, The Lilly Research Laboratories, Indianapolis, Indiana 46206 USA |
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Abstract: | Two small RNA viruses, mycophage PS-1, and bacteriophage φ6 were concentrated and purified by the sequential use of continuous flow zonal centrifugation, high speed continuous flow sedimentation, and combined rate zonal centrifugation and isopycnic banding. The continuous flow centrifugations were done using the K-II and K-XI rotors while the BXXIX rotor was used for batch rate zonal centrifugation and isopycnic banding. The deseribed procedures are useful for the concentration and purification of virus from large volumes of culture fluid. |
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