Thyrotropin-induced formation of functional follicles in primary cultures of ovine thyroid cells |
| |
| Authors: | W. W. Mak J. E. Errick R. C. Chan M. C. Eggo G. N. Burrow |
| |
| Affiliation: | 1. Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceara, Av. Maurocélio Rocha Ponte 100, postal code 62041-040, Sobral, CE, Brazil;2. Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraíba, Sousa, Paraíba, Brazil;3. Laboratory of Biochemistry and Gene Expression, Higher Institute of Biomedical Sciences, State University of Ceará, Fortaleza, CE, 60714-903, Brazil;4. Laboratory of Cell Biology, Cytology and Histology, Federal University of Vale do São Francisco (UNIVASF), Petrolina, Pernambuco, Brazil |
| |
| Abstract: | In primary cultures of ovine thyroid cells, TSH induced the expression of several differentiated functions including the formation of follicles, and synthesis and storage of iodinated thyroglobulin in the follicular lumen. In the present report, these follicles were shown by transmission (TEM) and scanning electron microscopy (SEM) to be intact, comprised of two or more cells and to possess numerous microvilli on the inner cell membranes facing the follicular lumen. The TSH-induced formation of follicles was reversible and dynamic, with the kinetics of formation preceding that of iodination. The follicles were further demonstrated to be functional in terms of thyroglobulin storage and iodination. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
