| Percoll等密度梯度离心法分离利什曼原虫细胞核 |
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| 引用本文： | 张建文,李靖炎.Percoll等密度梯度离心法分离利什曼原虫细胞核[J].动物学研究,1989,10(3):207-212. |
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| 作者姓名： | 张建文 李靖炎 |
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| 作者单位： | 中国科学院昆明动物研究所，中国科学院昆明动物研究所 中国医学科学院医学生物研究所 |
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| 摘 要： | 分离纯化锥虫类原生动物细胞核存在着许多困难,阻碍了人们对这类单细胞生物的细胞核在各方面作进一步研究。本文提出一个从破碎细胞开始的分离核的完整程序。采用起始密度为1.083的Percoll工作液作等密度梯度离心,获得了纯度较高的利什曼原虫(Leishmania gerbilli)细胞核。经测定,L.gerbilli细胞核在Percoll中的浮力密度为1.048—1.051。
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| 关 键 词： | 密度梯度离心 利什曼原虫 细胞核 |
ISOLATION AND PURIFICATION OF NUCLEI OF LEISHMANIA GERBILLI BY PERCOLL ISOPYCNIC DENSITY GRADIENTS CENTRIFUGATION |
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| Zhang Jianwen Li Jingyan.ISOLATION AND PURIFICATION OF NUCLEI OF LEISHMANIA GERBILLI BY PERCOLL ISOPYCNIC DENSITY GRADIENTS CENTRIFUGATION[J].Zoological Research,1989,10(3):207-212. |
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| Authors: | Zhang Jianwen Li Jingyan |
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| Abstract: | A complete procedure of nuclei isolation and purification from Leishmania gerbilli was established. With this procedure highly purified nuclei of L. gcrbilli can be obtained. The isopycnic density of the nuclei is about 1.048-1.051 in percoll media.
(Thc present address. Institute of Medical Biology, Chinese, Academy of Medical Sciences, Kunming, China.) |
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| Keywords: | Percoll isopycnic density gradients centrifugation Leishmania gerbilli Isolation and purification of nuclei |
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