Fuchsin fluorescence in Mycobacterium tuberculosis: the Ziehl-Neelsen stain in a new light |
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| Authors: | Shapiro Howard M Hänscheid Thomas |
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| Affiliation: | The Center for Microbial Cytometry and Howard M. Shapiro, M.D., P.C., 283 Highland Avenue, West Newton, MA 02465-2513, USA. hms@shapirolab.com |
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| Abstract: | Tuberculosis (TB) is often diagnosed by observation of reddish pink fuchsin-stained Mycobacterium tuberculosis (MTB) in Ziehl-Neelsen (Z-N) stained smears by transmitted light microscopy. MTB too faintly stained with fuchsin to be seen by transmitted light may be detected by their green-excited orange-red fluorescence; this finding may be clinically relevant. |
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| Keywords: | Fluorescence microscopy Fuchsin Tuberculosis diagnosis Ziehl– Neelsen stain |
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