Genomic organization, sequence analysis and expression of all five genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from tomato |
| |
Authors: | Mamoru Sugita Thianda Manzara Eran Pichersky Anthony Cashmore Wilhelm Gruissem |
| |
Affiliation: | (1) Department of Botany, University of California, 94720 Berkeley, CA, USA;(2) Department of Biology, University of Michigan, 48109 Ann Harbor, MI, USA;(3) Department of Biology, University of Pennsylvania, 19134 Philadelphia, PA, USA;(4) Present address: Department of Botany, Hokkaido University, 060 Sapporo, Japan |
| |
Abstract: | Summary We have cloned and sequenced all five members of the gene family for the small subunit (rbcS) of ribulose-1,5-bisphosphate carboxylase/oxygenase from tomato, Lycopersicon esculentum cv. VFNT LA 1221 cherry line. Two of the five genes, designated Rbcs-1 and Rbcs-2, are present as single genes at individual loci. Three genes, designated Rbcs-3A, Rbcs-3B and Rbcs-3C, are organized in a tandem array within 10 kb at a third independent locus. The Rbcs-2 gene contains three introns; all the other members of the tomato gene family contain two introns. The coding sequence of Rbcs-1 differs by 14.0% from that of Rbcs-2 and by 13.3% from that of Rbcs-3 genes. Rbcs-2 shows 10.4% divergence from Rbcs-3. The exon and intron sequences of Rbcs-3A are identical to those of Rbcs-3C, and differ by 1.9% from those of Rbcs-3B. Nucleotide sequence analysis suggests that the five rbcS genes encode four different precursors, and three different mature polypeptides. S1 nuclease mapping of the 5 end of rbcS mRNAs revealed that the mRNA leader sequences vary in length from 8 to 75 nucleotides. Northern analysis using gene-specific oligonucleotide probes from the 3 non-coding region of each gene reveals a four to five-fold difference among the five genes in maximal steady-state mRNA levels in leaves. |
| |
Keywords: | Tomato Nucleotide sequence Ribulose-1,5-bisphosphate carboxylase Multigene family Differential expression |
本文献已被 SpringerLink 等数据库收录! |
|