北部湾海洋原甲藻的形态特征及其系统进化分析

为探讨广西北部湾海洋原甲藻(Prorocentrum micans)的形态特征及其系统进化意义,利用光学显微镜、分子生物学方法,对海洋原甲藻BBW-01 藻株的形态特征进行了描述,并分析了其分子系统进化关系.结果表明,各地理株系的海洋原甲藻的形态特征相似,仅在细胞大小上存在差异.海洋原甲藻BBW-01 与采自于广东大亚湾的海洋原甲藻形态特征最为接近,其壳板后端的7 个呈“V”字形对称排列的大孔可作为海洋原甲藻鉴定的重要指标.18S rDNA 序列同源检索和系统进化分析表明,海洋原甲藻BBW-01 与源自中国南海的海洋原甲藻的亲缘关系最近,并与其他2 株海洋原甲藻聚成一支,属于浮游、兼性浮游类原甲藻.因此,对赤潮原因种的准确识别有助于预防和减轻赤潮对海洋环境和人类带来的危害.

Morphological and Phylogenetic Analysis of Prorocentrum micans Isolated from the Beibu Gulf

The aim was to understand morphological characteristics and phylogenetic evolution significant of Prorocentrum micans. The morphological characters of P. micans BBW-01strain, isolated from Beibu Gulf of Guangxi Province, was described under light microscopy, and phylogenetic evolution relationship was studied on the basis of molecular sequences. The morphological characters of different geographical P. micans tested in this study were similar, except of the range of cell size. The morphology of P. micans BBW-01 was more close to that isolated from Daya bay of Guangdong Province. Seven big pores arranged symmetrically with V-shape at the posterior end could be an important indicator of identification of P. micans. Homologous Blast and system evolution analysis on the basis of 18S rDNA sequence showed that P. micans BBW-01 had close relation with P. micans (DQ004735), and clustered together with two other P. micans, belonging to planktonic or facultative planktonic Prorocentrum. Therefore, the precise identification of the causative species was helpful to prevent and reduce the harm of red tide on marine environment and human beings.