| EB病毒相关胃癌中病毒分型的研究 |
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| 引用本文： | 杨婷婷,王云,刘霞,李欣,逄增昌,罗兵.EB病毒相关胃癌中病毒分型的研究[J].病毒学报,2009,25(1):29-34. |
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| 作者姓名： | 杨婷婷 王云 刘霞 李欣 逄增昌 罗兵 |
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| 作者单位： | 青岛大学医学院,微生物教研室,山东,青岛,266021;青岛市疾病预防控制中心,病原微生物与寄生虫检验科,山东,青岛,266033;青岛大学医学院,微生物教研室,山东,青岛,266021 |
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| 基金项目： | 国家自然科学基金(编号:30740068)资助 |
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| 摘 要： | 收集236例胃癌组织标本及135份健康人群咽漱液(throat washings,TWs)标本,采用原位杂交、PCR-Southern blot筛选出17例EB病毒相关胃癌(EBVaGC)(7.2%)和33例EBV阳性的TWs标本(24.4%);应用PCR-RFLP、巢式PCR及DNA测序等方法,检测EBV阳性标本病毒1/2分型、F/f分型、I/i分型及LMP1XhoI(+)/(-)等四种基因变异。EBVaGC及健康对照均为F型变异,未检测到f型变异。EBVaGC中1/2型、I/i型及LMP1XhoI(+)/(-)型的例数及比例分别为:17(100%)/0(0)、1(5.9%)/16(94.1%)及0(0)/15(88.2%);而TWs中上述分型的相应数据为25(75.8%)/8(24.2%)、11(33.3%)/19(57.6%)及12(36.4%)/18(54.5%),各位点两种基因型在EBVaGC和健康人中的分布不同(1/2:P=0.047;I/i:P=0.048;XhoI(+)/(-):P=0.012)。综合分析表明在3种基因多态性均能确定的标本中,EBVaGC均为1/i/XhoI(-)亚型(15/1...
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| 关 键 词： | EB病毒 胃癌 基因分型 |
Genotyping of Epstein-Barr Virus in Epstein-Barr Virus Associated Gastric Carcinoma |
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| YANG Ting-ting,WANG Yun,LIU Xia,LI Xin,PANG Zeng-chang,LUO Bing.Genotyping of Epstein-Barr Virus in Epstein-Barr Virus Associated Gastric Carcinoma[J].Chinese Journal of Virology,2009,25(1):29-34. |
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| Authors: | YANG Ting-ting WANG Yun LIU Xia LI Xin PANG Zeng-chang LUO Bing |
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| Institution: | 1.Department of Microbiology;Medical College of Qingdao University;Shandong Qingdao 266021;China;2.Department of Microbiological and Parasitological Laboratory;Qingdao Municipal Center for Disease Prevention and Control;Shandong Qingdao 266033;China |
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| Abstract: | Tumor samples from 236 gastric carcinoma patients and throat washings(TWs) from 135 healthy adults were collected and screened for EBV genome.17 Epstein-Barr virus associated gastric carcinoma(EBVaGC) and 33 EBV positive TWs were further examined for type 1/2 EBV and polymorphism at Bam HI F,Bam HI W1/I1 boundary regions and Xho I restriction site in LMP1 gene.No type f of Bam HI F polymorphism was found in all the cases,nor type 2 in EBVaGC.25 of 33 TWs(75.8%) analyzed detected type 1 virus,while 8 TWs(24.... |
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| Keywords: | Epstein-Barr virus gastric carcinoma genotypes |
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