Inheritance studies of SSR and ISSR molecular markers and phylogenetic relationship of rice genotypes resistant to tungro virus |
| |
Authors: | Mohammad Abdul Latif Mohammad Mahfuzur Rahman Mohammad Eaqub Ali Sadegh Ashkani Mohd Yusop Rafii |
| |
Institution: | 1. Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia;2. Bangladesh Rice Research Institute (BRRI), 1701 Gazipur, Bangladesh;3. Bangladesh Agricultural Research Institute (BARI), 1701 Gazipur, Bangladesh;4. Nanotechnology and Catalysis Research Center (NANOCEN), Universiti Malaya, 50603 Kuala Lumpur, Malaysia;5. Institute of Tropical Agriculture (ITA), Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia |
| |
Abstract: | Multivariate analyses were performed using 13 morphological traits and 13 molecular markers (10 SSRs and three ISSRs) to assess the phylogenetic relationship among tungro resistant genotypes. For morphological traits, the genotypes were grouped into six clusters, according to D2 statistic and Canonical vector analysis. Plant height, days to flowering, days to maturity, panicle length, number of spikelet per panicle, number of unfilled grain per panicle and yield were important contributors to genetic divergence in 14 rice genotypes. Based on Nei's genetic distance for molecular studies, seven clusters were formed among the tungro resistant and susceptible genotypes. Mantel's test revealed a significant correlation (r = 0.834*) between the morphological and molecular data. To develop high yielding tungro resistant varieties based on both morphological and molecular analyses, crosses could be made with susceptible (BR10 and BR11) genotypes with low yielding but highly resistant genotypes, Sonahidemota, Kumragoir, Nakuchimota, Khaiyamota, Khairymota and Kachamota. The chi-square analysis for seven alleles (RM11, RM17, RM20, RM23, RM80, RM108 and RM531) of SSR and five loci (RY1, MR1, MR2, MR4 and GF5) of three ISSR markers in F2 population of cross, BR11 × Sonahidemota, showed a good fit to the expected segregation ratio (1:2:1) for a single gene model. |
| |
Keywords: | Tungro resistant genotypes Genetic diversity Morphological traits Molecular markers Inheritance study |
本文献已被 ScienceDirect 等数据库收录! |
|