| 柞蚕蛹溶菌酶的分离和纯化 |
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| 引用本文： | 罗立新 黄自然. 柞蚕蛹溶菌酶的分离和纯化[J]. 工业微生物, 1997, 27(1): 21-24 |
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| 作者姓名： | 罗立新 黄自然 |
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| 作者单位： | [1]华南理工大学生物工程系 [2]华南农业大学蚕桑系 |
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| 摘 要： | 本文报道热空气，大肠杆菌Ｋ１２Ｄ３１诱导柞蛹血淋巴，提高溶菌酶活力的方法。应用选择性热变性，等电点沉淀，ＤＥＡＥ－纤维素离子交换层析，ＣＣ－纤维素亲和层析等分离技术从柞蚕蛹血淋巴中提取溶菌酶，溶菌酶比活力达３６７００ｕ／ｍｇ蛋白，提高了１５０倍，活力回收达４８．６％。
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| 关 键 词： | 柞蚕蛹 溶菌酶 分离 纯化 |
Isolation and Purification of Lysozyme from the Chinese Oak Silkworm Pupae |
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| Luo Lixin Yao Ruhua Huang Ziran. Isolation and Purification of Lysozyme from the Chinese Oak Silkworm Pupae[J]. Industrial Microbiology, 1997, 27(1): 21-24 |
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| Authors: | Luo Lixin Yao Ruhua Huang Ziran |
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| Abstract: | Induced by hot atmosphere and E. coli (K12D31), lysozyme extraction from immuned haemolymph was studied. Lysozyme was separated from pupae by following steps, heat denaturation, isoelectric precipitation, DEAE-cellulose ion-exchanged column chromatography and chitin-coated cellulose affinify chromatography the relative activity of the lysozyme preparation was 36700 units/mg protein, which was 150 times of the control. The total activity recovery rate was 48.6%. |
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| Keywords: | Chinese oak silkworm pupae Lysozyme Isolation Purification. |
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