Down-regulation of DNMT3b in PC3 cells effects locus-specific DNA methylation,and represses cellular growth and migration |
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| Authors: | Ahmed Yaqinuddin Sohail A Qureshi Romena Qazi Farhat Abbas |
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| Affiliation: | (1) Department of Biological & Biomedical Sciences, The Aga Khan University, Stadium Road, Karachi, 74800, Pakistan;(2) Department of Pathology & Microbiology, The Aga Khan University, Stadium Road, Karachi, 74800, Pakistan;(3) Department of Surgery, The Aga Khan University, Stadium Road, Karachi, 74800, Pakistan |
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| Abstract: | Background Aberrations in DNA methylation patterns promote changes in gene expression patterns and are invariably associated with neoplasia. DNA methylation is carried out and maintained by several DNA methyltransferases (DNMTs) among which DNMT1 functions as a maintenance methylase while DNMT3a and 3b serve as de novo enzymes. Although DNMT3b has been shown to preferentially target the methylation of DNA sequences residing in pericentric heterochromatin whether it is involved in gene specific methylation remains an open question. To address this issue, we have silenced the expression of DNMT3b in the prostate-derived PC3 cells through RNA interference and subsequently studied the accompanied cellular changes as well as the expression profiles of selected genes. |
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