Biodiesel production using a mixture of immobilizedRhizopus oryzae andCandida rugosa lipases |
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Authors: | Dong Hwan Lee Jung Mo Kim Hyun Yong Shin Seong Woo Kang Seung Wook Kim |
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Affiliation: | 1. Department of Chemical and Biological Engineering, Korea University, 136-701, Seoul, Korea
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Abstract: | Biodiesel conversion from soybean oil reached a maximum of 70% at 18 h using immobilized 1,3-specificRhizopus oryzae lipase alone. Biodiesel conversion failed to reach 20% after 30 h when immobilized nonspecificCandida rugosa lipase alone was used. To increase the biodiesel production yield, a mixture of immobilized 1,3-specificR. oryzae lipase and nonspecificC. rugosa lipase was used. Using this mixture a conversion of greater than 99% at 21 h was attained. When the stability of the immobilized lipases mixture was tested, biodiesel conversion was maintained at over 80% of its original conversion after 10 cycles. |
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