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Locked nucleoside analogues expand the potential of DNAzymes to cleave structured RNA targets |
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| Authors: | Birte Vester Lykke H Hansen Lars Bo Lundberg B Ravindra Babu Mads D Sørensen Jesper Wengel Stephen Douthwaite |
| Institution: | (1) The Nucleic Acid Center, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark;(2) The Nucleic Acid Center, Department of Chemistry, University of Southern Denmark, DK-5230 Odense M, Denmark;(3) Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen, Denmark |
| Abstract: | BackgroundDNAzymes cleave at predetermined sequences within RNA. A prerequisite for cleavage is that the DNAzyme can gain access to its target, and thus the DNAzyme must be capable of unfolding higher-order structures that are present in the RNA substrate. However, in many cases the RNA target sequence is hidden in a region that is too tightly structured to be accessed under physiological conditions by DNAzymes. |
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