Criteria for effective design, construction, and gene knockdown by shRNA vectors |
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| Authors: | Debra J Taxman Laura R Livingstone Jinghua Zhang Brian J Conti Heather A Iocca Kristi L Williams John D Lich Jenny P-Y Ting William Reed |
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| Institution: | (1) Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA;(2) Program of Molecular Biology and Biotechnology, University of North Carolina, Chapel Hill, NC 27599, USA;(3) Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA;(4) Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina, Chapel Hill, NC 27599, USA |
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| Abstract: | Background RNA interference (RNAi) technology is a powerful methodology recently developed for the specific knockdown of targeted genes.
RNAi is most commonly achieved either transiently by transfection of small interfering (si) RNA oligonucleotides, or stably
using short hairpin (sh) RNA expressed from a DNA vector or virus. Much controversy has surrounded the development of rules
for the design of effective siRNA oligonucleotides; and whether these rules apply to shRNA is not well characterized. |
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