Determination of bacteriocin activity with bioassays carried out on solid and liquid substrates: assessing the factor "indicator microorganism" |
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| Authors: | Maria Papagianni Nicholaos Avramidis George Filioussis Despina Dasiou Ioannis Ambrosiadis |
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| Affiliation: | (1) Department of Hygiene and Technology of Food of Animal Origin, Laboratory of Food Technology, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54006 Thessaloniki, Greece;(2) School of Mathematics, Faculty of Sciences, Aristotle University of Thessaloniki, 54006 Thessaloniki, Greece |
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| Abstract: | Background Successful application of growth inhibition techniques for quantitative determination of bacteriocins relies on the sensitivity of the applied indicator microorganism to the bacteriocin to which is exposed. However, information on indicator microorganisms' performance and comparisons in bacteriocin determination with bioassays is almost non-existing in the literature. The aim of the present work was to evaluate the parameter "indicator microorganism" in bioassays carried out on solid -agar diffusion assay- and liquid -turbidometric assay- substrates, applied in the quantification of the most studied bacteriocin nisin. |
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