Revision of the amino acid sequence of human heart fatty acid-binding protein |
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| Authors: | Torsten Börchers Peter Højrup Søren U. Nielsen Peter Roepstorff Friedrich Spener Jens Knudsen |
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| Affiliation: | (1) Department of Biochemistry, University of Münster, 4400 Münster, Germany;(2) Departments of Molecular Biology, University of Odense, 5230 Odense M, Denmark;(3) Department of Biochemistry, University of Odense, 5230 Odense M, Denmark;(4) Department of Medical Biochemistry, University of Aarhus, 8000 Aarhus C, Denmark;(5) Department of Biochemistry, University of Münster, Wilhelm-Klemm-Str. 2, D-4400 Mün-ster, Germany |
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| Abstract: | Summary Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP. The proteins were structurally analyzed by tryptic peptide mapping, application of plasma desorption time-of-flight mass spectrometry and amino acid sequencing. All three preparations of human heart FABP, having 132 amino acids, differed from the published sequence [Offner et al. Biochem J 251: 191–198, 1988] in position 104, where Leu is found instead of Lys, and in position 124, where Cys is found instead of Ser. |
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| Keywords: | fatty acid-binding protein mass spectrometry sequence human heart |
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