Istogenesi e caratteri anatomici dei tessuti ricavati da frammenti di germoglio di Solanum Tuberosum L. coltivati In Vitro

Abstract

HISTOGENESIS AND ANATOMICAL CHARACTERISTICS OF PORTIONS OF SOLANUM TUBEROSUM SPROUTS CULTIVATED IN VITRO. — Disks cut from sprouts of Solanum tuberosum L. have been cultured on White's basic or modified medium (White, 1943) with 20 mg/l KH2₂PO₄ 50 mg/l adenine, and 12 or 24 mg/l NAA.

Sections were prepared according to Morel's method (1948). Only on the disks cultured on White's modified medium with 12 mg/1/NAA growth glomerules and a normal meristematic layer were present, while there were no signs of cellular hypertrophy and organogenetic phenomena.

12 mg/l appears therefore to be the optimal dose of NAA for cultures of excised portions of potato sprouts. Adenine is however always necessary because it triplicates the growth activity of the tissues and because through its antagonism with regard to auxin it favours the genesis of the callus.