| Abstract: | In vitro interferon (IF) production in peritoneal macrophages of normal and Newcastle disease virus (NDV)-immunized mice was studied. Of ascites cells used, 80% were macrophages, 14% lymphocytes, and 6% polymorphonuclear leukocytes. It was indicated that IF was produced mainly in the macrophages after NDV inoculation. IF production in the macrophages derived from immunized mice was more enhanced than that in those from normal mice. It is not clear at present, however, whether this enhancement is based on immunological specificity. The IF production in the culture of macrophages reached its maximum value in 6 to 9 hr after inoculation of the inducer. After 12 hr, the IF titer in the culture fluid decreased gradually. A possible explanation of this fact is that there may be partial inactivation of IF by some cellular components. |
