Morphological and molecular diversity among populations of Quercus brantii Lindl. in western forest of Iran |
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Authors: | B Shiran S Mashayekhi H Jahanbazi A Soltani P Bruschi |
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Institution: | 1. Department of Agronomy and Plant Breeding, Faculty of Agriculture , Shahrekord University , Shahrekord, P.O. Box 115, Iran shiran@agr.sku.ac.ir or be_shiran@yahoo.com;3. Department of Agronomy and Plant Breeding, Faculty of Agriculture , Shahrekord University , Shahrekord, P.O. Box 115, Iran;4. Department of Forestry, Faculty of Natural Resources , Sari Agricultural Sciences and Natural Resources University , P.O. Box 578, Mazandaran, Sari, Iran;5. Department of Forestry, Faculty of Agriculture , Shahrekord University , Shahrekord, P.O. Box115, Iran;6. Dipartimento di Biotecnologie agrarie , Sezione di Botanica Ambientale ed Applicata, Piazzale delle Cascine , 28-I 50144, Firenze, Italy |
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Abstract: | Abstract This study represents a preliminary step toward understanding the genetic structure of Persian oak in Iran. The genetic variability of Quercus brantii in Western forest of Iran was evaluated by amplified fragment length polymorphism (AFLP), chloroplast microsatellite and leaf morphology. Fifty-five trees from eight regions were sampled from across the range of Chaharmahal va Bakhtiari province of Iran. Twenty morphological traits were analyzed through clustering and ordination method. At morphological level, the applied statistics suggest that macromorphological traits significantly differentiate between populations. The overall sample shows a proportion of AFLP polymorphic markers of 92.1%, denoting a high level of variability. Based on AFLP data, differences among populations within geographic regions account for 11.6% of the total variation and only 0.57% is attributed to variation among regions. Based on chloroplast microsatellite (cpSSR), 34% of total variation was found among populations, suggesting a high within-population haplotype diversity. The dendrogram obtained from cpSSR showed a general pattern quite different from the pattern obtained from morphological analysis and AFLP markers. |
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Keywords: | AFLP genetic variability macromorphological traits micromorphological traits microsatellite markers Quercus brantii |
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