Contenuto Dell'AIA Libero in Plantule di Vicia Sativa Cresciute in Diverse Condizioni di Ambiente. I. Plantule Cresciute Al Buio e Alla Luce

Abstract

Free beta-indoleacetic acid content in shoots of « Vicia sativa » seedlings grown in different environmental conditions. I. Seedlings grown in the dark and in the light. — Free IAA has been determined in shoots of Vicia sativa seedlings grown in the dark and in the light. The alcohol extract of the vegetable material has been submitted to several processes of purification (changes of PH and washing-out in ether, and successively column as well as thin layer chromatography). Lastly the IAA eluted with alcohol from the chromatographic slides is spectrometrically determined (λ = 280 mμ). The data show that the free IAA is contained to a greater extent in the seedlings grown in the dark in comparison to those grown in the light; this difference permaining in the samples of the same age as well as in those of the same height. The obtained data have been submitted to statistical analysis and both assays have shown to be highly significative.