A chimeric Potato virus X encoding a heterologous peptide affects Nicotiana benthamiana chloroplast structure |
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| Authors: | C Betti C Lico M Iriti S D’Angeli E Benvenuto S Baschieri |
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| Institution: | 1. Sezione di Genetica e Genomica Vegetale, ENEA C.R. , Casaccia, Italy;2. Dipartimento di Produzione Vegetale , Università di Milano , Italy;3. CNR , Istituto di Virologia Vegetale U.O. Milano , Italy;4. Dipartimento di Biologia Vegetale , Università “Sapienza” di Roma , Italy |
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| Abstract: | Abstract The cytopathology of a Potato virus X (PVX) recombinant variant (encoding as fusion of an epitope of immunological interest with the N‐terminus of the coat protein, PVXSmaP18DD) has been compared with that induced by the wild‐type virus (PVX wt) in Nicotiana benthamiana plants. Both PVX wt and PVXSmaP18DD caused similar ultrastructural alterations, characterized by the presence of laminated inclusion components and bulk virus accumulations in mesophyll cells. However, some striking differences were observed not only in the morphology of these accumulations (typically ordered in PVX wt infection and disordered in PVXSmaP18DD infection) but also because the chimeric virus caused peculiar alterations in chloroplasts structure. Abbreviations: CP, coat protein; d.p.i., days post inoculation; LIC, laminated inclusion components; PVX, Potato virus X |
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| Keywords: | Cytopathology flexiviridae LIC PVX coat protein |
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