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Three new hybrids of Ophioglossum (Ophioglossaceae) from Monte Pisano,Tuscany (Central Italy)
Authors:L Peruzzi  B Pierini  S Magrini  A Andreucci  D Marchetti  R Viane
Institution:1. Dipartimento di Biologia, Unità di Botanica, Università di Pisa, via Luca Ghini, 13 56126 Pisa, Italylorenzo.peruzzi@unipi.it;3. Dipartimento di Biologia, Unità di Botanica, Università di Pisa, via Luca Ghini, 13 56126 Pisa, Italy;4. Erbario e Banca del Germoplasma della Tuscia, Università della Tuscia – Largo dell'Università, 01100 Viterbo, Italy;5. Via Isonzo, 6 54100 Massa, Italy;6. Vakgroep Biologie, Ghent University, K. L. Ledeganckstraat, 35 – B-9000, Ghent, Belgium
Abstract:Based on general morphology, spore measurements and ornamentation (scanning electron microscope), genome size estimation, and molecular systematics (trnL-trnF IGS), we show the extreme systematic complexity within the European representatives of the genus Ophioglossum. In particular, three hybrids from Tuscany are described: the tetraploid O. × pierinii Peruzzi, Magrini, Marchetti & Viane, seen as the hybrid between diploid O. lusitanicum L. and hexaploid O. azoricum C.Presl; the tetraploid O. × giovanninii Peruzzi, Pierini, Magrini, Marchetti & Viane, seen as the homoploid hybrid between tetraploid O. vulgatum L. and tetraploid O. × pierinii Peruzzi, Magrini, Marchetti & Viane; the pentaploid O. × pseudoazoricum Peruzzi, Pierini, Magrini, Marchetti & Viane, seen as the hybrid between hexaploid O. azoricum C.Presl and tetraploid O. vulgatum L. All the three new taxa grow in different localities in the Monte Pisano mountain range.
Keywords:Flow-cytometry  genome size  hybridization  Ophioglossidae  Ophioglossum  Psilotopsida  SEM  taxonomy  trnL-trnF IGS
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