A simple device allowing UV-detection of the active substances of Silybum marianum (L.) Gaertner on TLC plates devoid of fluorescent indicator |
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| Authors: | Elsa M Cappelletti Renata Trevisan Rosamaria Caniato |
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| Institution: | Istituto di Botanica e Fisiologia Vegetale dell'Università , Via Orto Botanico 15, 35100, Padova |
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| Abstract: | Abstract A method for silybin, silydianin and silychristin (as well as taxifolin) visualization under long wave UV on silica gel TLC plates devoid of fluorescent indicator is described. This method has been devised because of the loss of the fluorescent indicator from TLC plates, when acid containing solvent systems are used. The method suggested takes advantage of the fluorescence light emitted by a fluorescent background, external to the plate and trasmitted through the plate itself. |
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