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Genetic transformation of Bacillus subtilis cells in the presence of clay minerals montmorillonite and kaolinite
Authors:Lotareva O V  Prozorov A A
Institution:Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow.
Abstract:The effect of the clay minerals montmorillonite and kaolinite on the transformation of competent Bacillus subtilis cells with chromosomal DNA was studied. Clay particles were found to substantially increase the transformation frequency of competent cells, as well as the rate of their spontaneous chromosomal and plasmid transformation. The effect was ascribed to the adsorption of bacterial cells on the surface of mineral particles.
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