Effect of carbamyl phosphate on the regulation of nitrogenase in Clostridium pasteurianum |
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Authors: | B Seto L E Mortenson |
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Institution: | Department of Biological Sciences, Purdue University, Lafayette, IndianaUSA |
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Abstract: | One mM carbamyl phosphate inhibited the acetylene reduction activity of nitrogenase 30% whereas at high concentrations a maximum inhibition of 50% was observed. When 1 mM carbamyl phosphate was added to a culture growing of N2 1) nitrogenase synthesis was completely repressed and 2) after a period of 2.5 hrs in the absence of growth, the specific activity decreased to less than 50% of its activity just before the addition of the inhibitor. |
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