The expression of granulocyte/macrophage colony-stimulating factor in activated mouse lymphocytes declines with age |
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| Authors: | N S Cai D D Li H T Cheung A Richardson |
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| Affiliation: | Department of Biological Sciences, Illinois State University, Normal 61761. |
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| Abstract: | The expression of granulocyte/macrophage colony-stimulating factor (GM-CSF) was studied in spleen lymphocytes isolated from male C57BL/6J mice of 6, 20, and 29 months of age. GM-CSF expression (biological activity and mRNA level) was maximum after culturing the lymphocytes for 45 hr with concanavalin A and phorbol myristate acetate. The induction of both GM-CSF activity and mRNA levels was observed to decline over 60% between 6 and 29 months of age. The age-related decline in the level of GM-CSF paralleled the age-related decline in the mRNA levels of interleukin-2 and interleukin-3. |
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