Asymmetric protoplast fusion between sweet potato and its relatives,and plant regeneration |
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Authors: | Marilyn M. Belarmino Toshinori Abe Takeo Sasahara |
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Affiliation: | (1) Philippine Root Crop Research and Training Center, ViSCA, 6521-A Leyte, Philippines;(2) Laboratory of Plant Breeding, Faculty of Agriculture, Yamagata University, 997 Tsuruoka, Japan |
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Abstract: | Experiments were conducted to asymmetrically fuse protoplasts from sweet potato (Ipomoea batatas L. Lam.) and its wild relativesI. trifida Don. andI. lacunosa L. Protoplasts of sweet potato were treated with iodoacetamide, whereas those ofI. trifida Don. andI. lacunosa L. were irradiated with X-rays. The asymmetric protoplast fusion was carried out by the electrofusion method and by polyethylene glycol treatment. Electrically-fused protoplasts initiated cell division, and then formed calli earlier than the polyethylene glycol-fused protoplasts. Plant regeneration occurred only in electrofused calli, suggesting that polyethylene glycol had some toxic effect on plant regeneration ability. Analysis of peroxidase isozymes confirmed the interspecific hybrid characteristics of both the fusion-derived calli and regenerated plants. |
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Keywords: | electrofusion iodoacetamide Ipomoea sweet potato polyethylene glycol peroxidase X-ray |
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