Luteinizing hormone-releasing hormone enhances polyphosphoinositide breakdown in rat granulosa cells |
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| Authors: | F Ma P C Leung |
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| Affiliation: | 1. Stem Cells and Metabolism Research Program, Faculty of Medicine, University of Helsinki, Helsinki, 00290, Finland;2. Molecular and Integrative Bioscience Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, 00790, Finland;3. Institute of Biotechnology, HiLIFE, University of Helsinki, Helsinki, 00790, Finland;4. Department of Neurosciences, Experimental Neurology and Leuven Brain Institute, KU Leuven – University of Leuven, 3000, Leuven, Belgium;5. VIB Center for Brain & Disease Research, Laboratory of Neurobiology, 3000, Leuven, Belgium;6. KU Leuven, Laboratory of Molecular and Cellular Signaling, Department of Cellular and Molecular Medicine & Leuven Kanker Instituut, Leuven, 3000, Belgium;7. Clinical Neurosciences, Neurology, University of Helsinki and Helsinki University Hospital, Helsinki, 00290, Finland;1. Department of Biomedical Sciences, University of Padova, Italy;2. Neuroscience Institute, National Research Council of Italy (CNR), Italy;3. Fondazione Istituto di Ricerca Pediatrica Città della Speranza (IRP), Italy;1. Department of Biochemistry and Molecular Biology, Structural Biology Imaging Center, McGovern Medical School at The University of Texas Health Science Center at Houston, 6431 Fannin Street, Houston, TX 77030, USA;2. Department of Pharmacology and Physiology, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA |
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| Abstract: | A 2-min addition of LHRH to [3H]inositol-prelabeled rat granulosa cells in primary culture evoked significant increases in the accumulation of [3H]inositol phosphates, i.e. radiolabeled inositol monophosphate (IP), inositol diphosphate (IP2), and inositol triphosphate (IP3) levels increased to 210, 590 and 520%, respectively, when compared to control cultures. By contrast, addition of FSH failed to elicit such a response. The effect of LHRH was completely blocked by the concomitant presence of a specific LHRH antagonist. LHRH evoked increase in [3H]IP3 and [3H]IP2 accumulation as early as 30 sec, while the increase in [3H]IP became significant at 2 min. These data support the hypothesis that polyphosphoinositide breakdown may be an early step in the intracellular signal mechanism which mediates the action of LHRH. |
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