骨痹消对兔骨性膝关节炎软骨细胞p53、Bcl-2表达的影响

目的探讨骨痹消对兔实验性膝关节骨性关节炎(KOA)的治疗作用。方法参照文献1]介绍的伸膝制动方法复制KOA动物模型,并随机分为正常组(A组)、模型组(B组)、骨痹消组(C组),治疗6周、10周后采用免疫组化技术测各组软骨细胞p53、Bcl-2表达。结果与正常组比较,模型组Bcl-2、p53表达增高,且其表达水平随着病程(6w、10w)呈正相关,骨痹消组用药6w及10w时Bcl-2的表达随时间的延长呈逐渐增强的趋势,较模型组相比呈显著性差异(P0.01),同时p53的阳性表达明显下降,与模型组相比均呈显著性意义(P0.01)。结论抑凋亡(APO)基因Bcl-2和促APO基因p53共同参与了软骨细胞APO的调节,骨痹消可通过上调Bcl-2、下调p53表达,以保护软骨细胞,防止发生APO。

EFFECTS OF GUBIXIAO ON THE EXPRESSIONS OF P53 AND BCL-2 GENE IN KNEE OSTEOARTHRITIS CHONDROCYTES

Objective To explore the therapeutical effects of gubixiao on KOA of the empirical rabbits.Methods 36 Rabbits were randomly divided into three groups：control group,model group,gubixiao group,After being treated for 6 weeks and 10 weeks,The expression rate of p53mRNA and Bcl-2mRNA of cartilage cell of all groups of rabbits were measured.Results the expression of p53mRNA and Bcl-2mRNA of cartilage cell of model group increased,the level of expression are positive correlations.After being treated,the expression of Bcl-2mRNA of gubixiao Group significantly increases compared with that of model group（P〈0.01）.The expression of p53mRNA of gubixiao group obviously decreased compared with that of model group（P〈0.01）.Conclusion Bcl-2 and p53 Involve Regulation of Chondrocytes APO,up-regulating the protein expression of Bcl-2,down-regulating the protein expression of Bcl-2,protect chondrocytes,prevent APO.