An automatic image analysis system for RLGS films |
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| Authors: | Yuichi Sugahara Shingo Akiyoshi Yasushi Okazaki Yoshihide Hayashizaki Isao Tanihata |
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| Institution: | (1) Genome Science Laboratory, RIKEN Tsukuba Life Science Center, 3-1-1, Koyadai, Tsukuba, Ibaraki 305, Japan, JP;(2) Linac Laboratory, RIKEN, 2-1, Hirosawa, Wako, Saitama 351-01, Japan, JP;(3) CREST, JST, 4-1-8, Honcho, Kawaguchi, Saitama 322, Japan, JP |
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| Abstract: | The RLGS (Restriction Landmark Genome Scanning) method was originally developed as a powerful method for enabling viewing
of thousands of restriction landmarks. It offers a tool for obtaining information about genetic loci, with a single RLGS profile
displaying approximately 2000 restriction landmarks as spots. One of the most useful applications is RLGS spot mapping, which
allows the efficient, low-cost construction of the genetic map of any organism. However, analyses of the profiles depend mainly
on human visual observation and are tedious and laborious. Although several commercially available image analyzing systems
for profile comparison have been examined, they cannot be used for the RLGS spot mapping system owing to the background characteristics
of the RLGS profiles, unsatisfactory rates of correspondence, and inefficient correction of informative genetic data. We therefore
developed a novel automatic image analysis system for RLGS spot mapping, using an original algorithm based on the binary image
transferred from the original RLGS profile. This system was employed for identifying non-polymorphic and parental strain-specific
polymorphic spots of the F1 mouse profile and yielded efficient initial screening of RLGS profiles.
Received: 5 December 1997 / Accepted: 6 April 1998 |
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