Inhibition of somatic embryo maturation in Sitka spruce [Picea sitchensis (Bong.) Carr.] by butylated hydroxytoluene,a volatile antioxidant released by parafilm |
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| Authors: | Chris Selby W. Colin McRoberts John T. G. Hamilton Barbara M. R. Harvey |
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| Affiliation: | (1) Applied Plant Science Division, The Queen's University of Belfast, Newforge Lane, BT9 5PX Belfast, UK;(2) Food Science Division, Department of Agriculture for Northern Ireland, The Queen's University of Belfast, Newforge Lane, BT9 5PX Belfast, UK;(3) Department of Applied Plant Science, The Queen's University of Belfast, Newforge Lane, BT9 5PX Belfast, UK |
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| Abstract: | Head-space volatiles above embryogenicPicea sitchensis (Bong.) Carr. (Sitka spruce) tissues cultured in glass Petri dishes sealed with Parafilm M or cling-film, were captured on Tenax adsorption traps and analysed by gas chromatography / mass spectrometry. Each sealing system released a single major compound into the head-space; butylated hydroxytoluene from Parafilm M and 2-ethyl-1-hexanol from cling-film. After two weeks sealed under Parafilm M butylated hydroxytoluene accumulated to 1.1  g g–1 FW in tissues and subsequent somatic embryo maturation was prevented. When butylated hydroxytoluene was supplied via the head-space (100 g/250 ml flask) 0.5 g g–1 FW accumulated in tissues after two weeks and no somatic embryo maturation occurred. Potentially phytotoxic metabolites of butylated hydroxytoluene included a substituted stilbenequinone, butylated hydroxytoluene quinone methide and butylated hydroxytoluene dimer. |
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