A rapid and sensitive method for HPLC cholesterol determination in bile. |
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| Authors: | C Bocos M Castro E Orozco J A Contreras E Herrera |
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| Affiliation: | Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, Hospital Ramón y Cajal, Madrid, Spain. |
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| Abstract: | A relatively little time consuming simple method based on the treatment of bile with cholesterol oxidase and subsequent high performance liquid chromatography measurement of the 3-ketocholesterol produced in order to determine the level of the cholesterol concentration is described. The method avoids bilirubin interferences, has high reproducibility and recovery assays give 100% values. It is highly sensitive and suitable for use in the determination of cholesterol concentrations in bile and other bilirubin containing biological fluids. |
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