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The structure of the carbohydrate backbone of the LPS from Shewanella putrefaciens CN32
Authors:Vinogradov Evgeny  Korenevsky Anton  Beveridge Terry J
Institution:Institute for Biological Sciences, National Research Council, Ottawa, Ont., Canada. evguenii.vinogradov@nrc.ca
Abstract:The lipopolysaccharide (LPS) from a natural rough strain of Shewanella putrefaciens CN32 was analyzed using NMR and mass spectroscopy and chemical methods, and the following structure of its carbohydrate backbone is proposed: beta-Galf-(1-->3)-beta-Gal-(1-->4)-beta-Glc-(1-->4)-alpha-DDHep2PEtN-(1-->5)-alpha-Kdo4P-(1-->6)-beta-GlcN4P-(1-->6)-alpha-GlcN1P
Keywords:LPS  lipopolysaccharide  DDHep  d-glycero-d-manno-heptose" target="_blank">d-glycero-d-manno-heptose  Kdo  d-manno-octulosonic acid" target="_blank">3-deoxy-d-manno-octulosonic acid  P  phosphate  PEtN  phosphoethanolamine  HPAEC  high-performance anion-exchange chromatography
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