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Rapid in vitro propagation of cauliflower |
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| Authors: | Atul Kumar Vandana A Kumar and Jitendra Kumar |
| Institution: | G.B. Pant University of Agriculture and Technology, Hill Campus, Ranichauri, Dt. Tehri Garhwal, India |
| Abstract: | A rapid single step method for complete plantlet regeneration in cauliflower is described. Curd explants cultured on Murashige and Skoog (MS) medium supplemented with 1 mg−1 indoleacetic acid (IAA) developed complete plantlets in 25 days. Regeneration potential of curd explants was drastically reduced with increasing storage of cauliflower curds at room temperature beyond 8 days of harvesting. Light intensity of 3000 lux supplied by white fluorescent tubes was optimum to evoke the best response. It was possible to field transfer the hardened plantlets within 35 days of culture initiation. |
| Keywords: | clonal cauliflower curd protocol |
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