Identification of a phi X174 coded protein involved in the inhibition of beta-galactosidase synthesis in Escherichia coli |
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Authors: | S K Pal R K Poddar |
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Affiliation: | Biophysics Section, Department of Physics, University College of Science, 92 Acharya Prafulla Chandra Road, Calcutta-700 009 India. |
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Abstract: | The synthesis of β-galactosidase (EC 3.2.1.23: β-D-galactoside galactohydrolase) in is repressed as a result of infection with single-stranded DNA phage ØX174. An mutant in ØX174 cistron A, which codes for two proteins, does not inhibit the enzyme synthesis while mutants in all other genes do cause repression. A mutant near the amino-terminal end of cistron A, which produces the small 35,000 molecular weight cistron A polypeptide, also inhibits the synthesis of β-galactosidase. Inhibition is also observed in an mutant which does not support the replication of replicative-form DNA. Exogenous nucleotide bases and cyclic 3′,5′-adenosine monophosphate (cyclic AMP) do not have any effect on the degree of repression. |
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