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Ribosomal DNA distribution in somatic chromosomes of Stangeria eriopus (Stangeriaceae, Cycadales) and molecular-cytotaxonomic relationships to some other cycad genera
Authors:Goro Kokubugata  Ken D Hill  Katsuhiko Kondo
Institution:(1) Tsukuba Botanical Garden, National Science Museum, Tokyo, Amakubo 4, 305-0005 Tsukuba, Ibaraki, Japan;(2) National Herbarium of New South Wales, Royal Botanic Gardens, Sydney, Mrs Macquaries Rd., 2000 Sydney, New South Wales, Australia;(3) Laboratory of Plant Chromosome and Gene Stock, Graduate School of Science, Hiroshima University, Higashi-hiroshima, 739-8526 Hiroshima, Japan
Abstract:Somatic chromosomes ofStangeria eriopus (Stangeriaceae, Cycadales) were investigated by fluorescentin situ hybridization (FISH) using an 18S ribosomal DNA (rDNA) probe.Stangeria eriopus showed a chromosome number of 2n=16 with a karyotype of 12 median-, 2 subterminal-, and 2 terminal-centromeric chromosomes. FISH study ofS. eriopus revealed 16 signals made up of rDNA sites located on the terminal regions of the long arms of the 7 median- and 2 subterminal-centromeric chromosomes, on terminal region of the short arm of the 1 median-centromeric chromosome, on the terminal regions of the long and the short arms of 1 median- and 2 terminal-centromeric chromosomes. This result suggests that, not only karyomorphologically but also molecular-cytologically, the genusStangeria may be more closely related to the genusCeratozamia than the genusBowenia or the genusMicrocycas previously hypothesized.
Keywords:Stangeria            Cycadales  chromosome  FISH  ribosomal DNA
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