| 宇佐美曲霉L336酸性蛋白酶的动力学性质* |
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| 引用本文： | 翁醒华 徐晨明 李永泉 胡华萃 周红军 贺筱蓉. 宇佐美曲霉L336酸性蛋白酶的动力学性质*[J]. 菌物学报, 1999, 18(1) |
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| 作者姓名： | 翁醒华 徐晨明 李永泉 胡华萃 周红军 贺筱蓉 |
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| 作者单位： | 杭州大学生命科学学院 杭州 310028 |
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| 摘 要： | L336酸性蛋白酶的分子量为54000，等电点为3．8±0．2。在pH2．5，40℃条件下，酶对酪蛋白、牛血清白蛋白和牛血红蛋白的Km值分别为0．263g／L、0．278g／L和0．415g／L，Vmax分别为2075、1550和2793μgTyr／min、mL，酪蛋白为最适底物。在pH2．0～5．0、40℃以下时酶的稳定性最好。此外，还研究了金属离子对酶活性的影响。
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| 关 键 词： | 霉菌 酸性蛋白酶 动力学性质 |
STUDIES ON THE KINETIC PROPERTY OF ACID PROTEINASE FROM ASPERGILLUS USAMII MUTANT L336 |
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| Abstract: | The L336 acid proteinase from Aspergillus usamii was shown to have a molecular weight of 54000 and an isoelectric point of 3.8± 0.2. The Km Values of the enzyme at 40℃ and pH2.5 for casein、bovine serum albumin and bovin haemoglobine were 0.263g / L. 0.278g / L and 0.415g / L reapectively, and the Vmax values under the same conditions were 2075. 1550 and 2793μg Tyr / min、mL. The optimum substrate for acid proteinase was casein. The enzyme was stable at temperature up to 40℃ and pH2.5~5.0. In addition, the effect of different metal ions on the activities of enzyme was also studied. |
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| Keywords: | Aspergillus Acid proteinase Xinetic property |
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