Proteome profiling of heat shock of human primary breast epithelial cells,a dataset report |
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Authors: | Min Jia Serhiy Souchelnytskyi |
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Institution: | (1) Department of Oncology-Pathology, Karolinska Biomics Center, Karolinska Institutet, SE-17176, Stockholm, Sweden |
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Abstract: | Exposure to elevated temperatures has a strong effect on cell functions, and is used in clinical practice. Hyperthermia may
affect multiple regulatory mechanisms in cells. To understand better the response to hyperthermia of immortalized primary
human breast epithelial cells, we performed a proteomics study of these cells cultured at 34°C or 39°C. Twenty-four proteins
were shown to be differentially expressed due to hyperthermia. Analysis of these proteins showed the potential involvement
of various biological processes in response to hyperthermia, e.g., cell adhesion, cell communication, and cell cycle. Transforming
growth factor-β2 (TGF-β2) and heat shock protein 27 (HSP27) were found to be upregulated at 39°C. TGF-β2 was found to affect
expression of HSP27, and to have a protective role in hyperthermia-induced cell death. Thus, the dataset described here of
hyperthermia-related proteins in human primary breast epithelial cells predicts a number of cellular activities affected by
exposure to high temperatures and provides a set of proteins for further studies. |
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Keywords: | Heat shock response Proteomics Systemic analysis Breast primary epithelial cell Transforming growth factor-β Heat shock protein 27 |
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