Antioxidant capacities in various animal sera as measured with multiple free-radical scavenging method |
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| Affiliation: | 1. Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory for Agro-Ecological Processes of Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China;2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;3. Department of Animal Nutrition, Federal University of Agriculture, P.M.B. 2240, Abeokuta, Ogun State, Nigeria;4. Nutrition Research Division, Food Directorate, Health Canada, Ottawa, Ontario K1A0K9, Canada;1. College of Science, Hebei University of Engineering, Handan 056038, China;2. Department of Composite Materials and Engineering, College of Equipment Manufacturing, Hebei University of Engineering, Handan 056038, China;1. Department of Mathematical Sciences, Tsinghua University, Beijing 100084, PR China;2. Department of Biological Sciences, Chicago State University, Chicago, IL 60628, USA |
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| Abstract: | Scavenging abilities of animal sera against six reactive species (OH , O2−, RO, t-BuOO, H3C, and 1O2) were determined with the use of multiple free-radical scavenging (MULTIS) method. Commercially available sera from pig, horse, rabbit, Guinea pig, hamster and chicken were subjected to MULTIS analysis and the results were compared with human specimen. In general, animal sera showed lower scavenging ability against OH and RO radicals than human serum. However, it is noteworthy that rabbit and chicken sera have higher scavenging ability against O2− than others. This is consistent with the known data that superoxide dismutase levels in these sera are high. In addition, we determined the uric acid level in animal sera using the uricase-TOOS method. In chicken serum, uric acid was found to be the major effective component in RO scavenging. This paper is first to quantitatively evaluate antioxidant capacities in animal sera. |
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| Keywords: | Animal serum Uric acid Antioxidant ability Multiple radical scavenging ESR spin-trapping |
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