Highly sensitive fluorescence imaging of cancer with avidin-protease probe conjugate |
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Affiliation: | 1. Department of Biomedical Engineering, University of Florida, Gainesville, FL;2. Department of Surgery, Emory University School of Medicine, Atlanta, GA |
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Abstract: | It is a long-term goal of cancer diagnosis to develop tumor-imaging techniques that have sufficient specificity and sensitivity to detect small tumor nodules during surgery or endoscopic surgery. Here, we introduce an avidin-conjugated fluorescence probe, Avidin-Leu-HMRG, which consists of a cancer-targeting macromolecule (avidin) and a protease-activatable probe. The conjugate has a high affinity for lectin on cancer cells and undergoes endocytosis, followed by irreversible fluorescence activation due to cleavage by lysosomal leucine aminopeptidase. In a mouse model of peritoneal ovarian metastases, the probe could detect submillimeter-sized tumor nodules with a high S/N ratio at 1 h after intraperitoneal injection. |
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Keywords: | Cancer imaging Fluorescence probes Avidin Lysosomal protease Click chemistry |
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