Self-renaturing fractions in the separated strands of mouse satellite deoxyribonucleic acid |
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Authors: | W D Sutton and P M B Walker |
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Institution: | Medical Research Council Group on the Mammalian Genome, Department of Zoology, University of Edinburgh, West Mains Road, Edinburgh EH9 3JT, U.K. |
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Abstract: | Pyrimidine- and purine-rich strands of Mus musculus satellite DNA prepared by alkaline CsCl-gradient centrifugation can self-renature to a variable extent to give partial duplexes with high thermal stability. These duplexes were purified by treatment with nuclease S(1) followed by hydroxylapatite chromatography, and have been shown by pyrimidine-tract analysis to be very similar in sequence to total reassociated satellite DNA. It is believed that the self-renaturing fractions result from variable contamination of each strand with fragments of the other, rather than from molecular inversions. The predominantly single-stranded properties of these fractions may be partly due to the ability of mouse satellite DNA strands to reassociate in non-stoicheiometric proportions. |
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