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A common precursor for the two subunits of the penicillin acylase from Escherichia coli ATCC11105 |
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| Authors: | G Oliver F Valle F Rosetti M Gómez-Pedrozo P Santamaría G Gosset F Bolivar |
| Institution: | Depurtamento de Genética y Biologia Molecular, Centra de Investigación sobre Ingeniería Genética y Biotecnologia Universidad Nacional Autónoma de México, Cuernavaca, MorelosMéxico Tel. (5273) 172399 |
| Abstract: | Penicillin acylase (PA) is an industrial enzyme that is used to convert penicillin G into a precursor for semisynthetic penicillins. We have cloned a segment of DNA that codes for the two subunits required for PA activity. We also report the nucleotide sequence of a DNA fragment that codes for (i) the small subunit, (ii) the N-terminal region of the large subunit and (iii) a putative connecting peptide. These results confirm the existence of a common precursor for both peptides. |
| Keywords: | Recombinant DNA protein processing leader peptide DNA sequence pBR339 vector aa amino acid(s) 6-APA 6-amino penicillanic acid bp base pair(s) DMBA kb 1000 bp LB Luria broth nt nucleotide(s) ORF open reading frame PA penicillin acylase SD Shine-Dalgarno |
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