Permanent Preservation of Whole Alizarin Red S Skeletons by Clearing and Embedding in Polyester Resins |
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| Authors: | Daniel Chappard Christian Alexandre Sabine Palle Georges Riffat |
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| Affiliation: | a L.B.T.O. Laboratory of Bone Biology, GIP “Exercise” North Hospital, University of Saint Etienne, Saint-Priest-en-Jarez, France |
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| Abstract: | A one-step clearing and embedding procedure for alizarin red S stained skeletons is described. Embryos are fixed in formalin, skinned and eviscerated. After staining in a 10 mg/liter solution of alizarin red S in 5% aqueous KOH, specimens are dehydrated in a graded series of acetone-polyester monomer solutions. Finally, the specimens are embedded at room temperature in the polyester resin. A special reusable metallic mold is described for embedment of large fetuses. Specimens previously cleared in glycerol can be processed with this method. |
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