Microarray analysis of tumour antigen expression in presentation acute myeloid leukaemia |
| |
Authors: | Guinn Barbara-Ann Gilkes Amanda F Woodward Eleanor Westwood Nigel B Mufti Ghulam J Linch David Burnett Alan K Mills Ken I |
| |
Institution: | Department of Haematological Medicine, Guy's, King's and St. Thomas' School of Medicine, King's College London, London SE5 9NU, UK. barbara.guinn@kcl.ac.uk |
| |
Abstract: | Acute myeloid leukaemia (AML) is a difficult to treat disease, especially for those patients who have no eligible haematopoietic stem cell (HSC) donor. One of the most promising treatment options for these patients is immunotherapy. To investigate the expression of known tumour antigens in AML, we analysed microarray data from 124 presentation AML patient samples and investigated the present/absent calls of 82 tumour-specific or -associated antigens. We found 11 antigens which were expressed in AML patient samples but not normal donors. Nine of these were cancer-testis (CT) antigens, previously shown to be expressed in tumour cells and immunologically protected sites and at very low levels, if at all, in normal tissues. Expression was confirmed using real-time PCR. We have identified a number of CT antigens with expression in presentation AML samples but not normal donor samples, which may provide effective targets for future immunotherapy treatments early in disease. |
| |
Keywords: | Microarray Cancer-testis antigens Acute myeloid leukaemia RAGE1 FLT3 |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|