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Rapid Detection of Banana Streak Virus by Loop‐mediated Isothermal Amplification Assay in South China |
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| Authors: | Jun Peng Zaifeng Fan Junsheng Huang |
| Institution: | 1. Authors’ addresses:Environmental and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences;2. Key Laboratory of Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests;3. Danzhou, Hainan 571737, China;4. State Key Laboratory of Agro‐biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing 100193, China (correspondence to J. Huang. E‐mail: h888111@126.com);5. State Key Laboratory of Agro‐biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing 100193, China (correspondence to J. Huang. E‐mail: h888111@126.com) |
| Abstract: | Banana streak virus (BSV) is a significant constraint to banana production and genetic improvement. It is necessary to develop and use BSV detection strategies that are both reliable and sensitive for the management of the virus. A loop‐mediated isothermal amplification (LAMP) assay was developed and evaluated for the detection of BSV. Four primers matching a total of six sequences of the conserved ORF III polyprotein genes were synthesized for developing a specific and sensitive LAMP for DNA extracts from field‐infected banana plants. LAMP assay could detect as low as 1 pg/μl template DNA. Test results of all field samples collected from different regions of South China showed that LAMP is more sensitive than PCR. This relatively simple and sensitive technique showed excellent potential with field‐collected samples and for routine screening of tissue culture materials in South China. |
| Keywords: | Banana streak virus LAMP virus detection and management China |