Characterization and Complete Nucleotide Sequence of Two Isolates of Tomato mosaic virus |
| |
| Authors: | Qiong Hu Tong Jiang Chaoyang Xue Xueping Zhou |
| |
| Affiliation: | 1. Authors’ addresses: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, 310029, China;2. School of Plant Protection, Anhui Agricultural University, Hefei 230036, China (correspondence to X. Zhou. E‐mail: zzhou@zju.edu.cn) |
| |
| Abstract: | Two virus isolates, designated S1 and TL, were obtained from tomato and camellia root in China, respectively, and their host ranges, symptomatology, serological reactions and complete nucleotide sequences were determined. Isolate TL systemically infected Chenopodium amaranticolor causing leaf chlorosis, but the isolate S1 induced only local necrotic lesions. The complete nucleotide sequences of S1 and TL were determined and consisted of 6384 and 6383 nucleotides (Genbank accessions AJ132845 and AJ417701 ), respectively. Sequence analysis revealed that both isolates have the highest nucleotide sequence identity (over 92%) with Tomato mosaic virus (ToMV), but less (80%) with other tobamoviruses. Phylogenetic analyses based on the amino acid sequences of 30‐kD and 17.5‐kD proteins also indicated that both the isolates form a cluster with the isolates of ToMV. These data suggest that S1 and TL are isolates of ToMV. The possible reasons that TL infected C. amaranticolor systemically but S1 induced only local necrotic lesions are discussed. |
| |
| Keywords: | Tomato mosaic virus characterization nucleotide sequence China |
|
|
